- 100 grams H2O
- 1.5 grams agar
- 1.5 grams Fermaid K
- 12 grams light DME
Since I don't want to deal with mixing up my media at work I am breaking the rules of sterility and mixing it up stove top. I dissolved the solids in hot H20 and boiled in a sterile 250 ml beaker on my stovetop. This should be good enough to kill any yeasts and most bacteria (except for the spore-formers). If I was using these plates for a commercial application I might be concerned but since they are for my own use I'm taking the"Relax, Don't Worry, Have A Homebrew!" approach. I will let the plates sit for several days to a week to look for any growth to indicate contamination.
After finishing I realized I might want to make up some more plates to reculture out some of my existing plates since they are getting a little old. Well one thing at a time....